The David H. Murdock Research Institute:

Core Technologies…Countless Possibilities

Built from the ground up to serve, facilitate, and overcome some of science’s biggest challenges, the David H. Murdock Research Institute will truly be a one-of-a-kind research facility. The DHMRI will host a diverse collection of the world’s most advanced research equipment – all conveniently located under one roof. A dedicated multidisciplinary team will be available at the DHMRI to help educate, challenge, and assist investigators in applying the most suitable technologies to their research programs. For the first time, scientists will be able to tap into these capabilities as if they were their own.

A broad range of multidisciplinary technologies will be available at the DHMRI:

• Genomics - The Molecular Genomics Laboratory (MGL) provides state-of-the-art support to researchers who require DNA sequencing, genotyping and/or gene expression services. In addition, DNA and RNA extraction and purification will be available.
  
  
• Metabolomics – The Metabolomics Laboratory provides methods for targeted and broad spectrum analysis utilizing nuclear magnetic resonance (NMR) and mass spectrometry (MS)-based technologies. Through the linking of metabolites to known biochemical pathways, investigators can develop mechanistic insights that show promise in staging disease, monitor therapeutic treatments, and develop or improve intervention strategies.
  
  
• Proteomics - The Proteomics Laboratory will offer the most up-to-date proteomics equipment available for the detection and identification of biomarkers. Featured will be the latest equipment for protein separation and state-of-the-art mass spectrometers for detecting and quantifying proteins in a sample.
  
  
• NMR – The NMR Laboratory will house one of the world's first actively-shielded 950 MHz NMR superconducting magnet in conjunction with 700 MHz and 600 MHz NMRs. The NMR laboratory will provide the most modern capabilities with applications in biology, biochemistry, toxicology, and synthetic chemistry.
  
  
• Microscopy – The Integrated Microscopy Laboratory (IML) will provide researchers access to the equipment and expertise required to examine cellular and subcellular details at high resolution by light, laser, and electron microscopy – including transmitted light, widefield fluorescence structured illumination, confocal and multi-photon laser scanning microscopy, fluorescence correlation spectroscopy (FCS) and total internal reflection fluorescence (TIRF) microscopy. Also available will be cryo-transmission electron microscopy (cryo-TEM), cryo-scanning electron microscope (SEM), and sophisticated image analysis.
  
  
• Clinical Discovery - The Clinical Discovery Laboratory provides high-quality flow cytometry and multi-parameter cell sorting. Resources are also available for performing immunoassay, multiplex assays, and clinical chemistry analysis covering a wide range of clinically important parameters ranging from electrolytes to cardiac markers to tumor antigens.
  
  
• Histochemistry - The Histochemistry Laboratory provides resources to examine the chemical components of cellular and subcellular tissue ranging from basic histology to complex immunostaining. The laboratory will also provide microscope slide-based laser microdissection and the capability to capture pure samples of RNA, DNA, proteins, and living cells from fresh tissue, as well as mosaic imaging capabilities.
  
  
• Cell Culture - Routine uses of the Cell Culture Laboratory will include hybridoma generation for monoclonal antibody production, transgenic and knock-out mouse production, as well as, support for gene expression studies, cell sorter procedures, preparation of cell extracts, cell transformation studies, live-cell imaging, reporter gene assays, DNA immortalization, toxicology studies.
  
  
• Transgenics - The Transgenic Mouse Facility will provide the capabilities to produce transgenic mice by microinjection of foreign DNA into fertilized mouse embryos.

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